Do shrooms show up on a drug test?
If you’re working under DOT regulations or for a zero-tolerance employer, this question is more relevant than ever in 2025. With psilocybin (the active compound in “shrooms”) gaining attention in both therapy and recreation, many employees wonder: Can I get caught during a drug test?
Let’s break it down.
What Are Shrooms?
Shrooms are psychedelic mushrooms containing psilocybin, a mind-altering substance. While a few U.S. states have decriminalized psilocybin, it remains federally illegal and classified as a Schedule I drug.
DOT-covered workers (CDL drivers, airline staff, etc.) are still subject to federal laws, regardless of state-level legalization.
Do Shrooms Show Up on a Drug Test in 2025?
Here’s the short answer:
👉 Standard DOT drug tests do not test for shrooms, but specialized tests can.
DOT’s Standard 5-Panel Test Screens for:
Marijuana (THC)
Cocaine
Opiates (heroin, codeine, morphine)
Amphetamines (including meth)
PCP
So, psilocybin (shrooms) is not part of the default DOT panel in 2025.
BUT employers can request extended panels that include psilocybin—especially if:
You self-disclose psychedelic use
You show signs of impairment
It’s a high-security or zero-tolerance job
Detection Windows for Psilocybin
| Test Type | Detection Window |
|---|---|
| Urine (basic) | 1–3 days (rarely detects shrooms) |
| Urine (advanced) | Up to 7 days |
| Blood | Up to 24 hours |
| Hair Follicle | Up to 90 days (advanced labs) |
Pro Tip: Just because most employers don’t test for it doesn’t mean they won’t.
Will Shrooms Affect Your Job or CDL?
Yes—even without a positive test.
Under DOT policy, you can still face:
Suspension or termination
Mandatory SAP Evaluation
Return-to-Duty process
If your employer suspects impairment or has a zero-tolerance policy, shroom use could result in loss of safety clearance.
What Happens If You Admit to Shroom Use?
You may be required to:
Undergo a DOT-qualified SAP Evaluation
Complete education or treatment
Pass a Return-to-Duty test
👉 Start Your SAP Evaluation Now
Legal ≠ Allowed at Work
Even if your state has decriminalized shrooms:
DOT regulations still apply
Federal employers and contractors follow federal law
You can be removed from duty even without a failed test
Key Takeaways
Do shrooms show up on a drug test? Not always—but they can.
Standard DOT drug panels in 2025 don’t test for psilocybin.
Employers can use expanded panels or act on behavioral suspicions.
Use under federal employment may lead to disciplinary action or mandatory SAP treatment.