Can Psilocybin Be Detected in Drug Tests? The Truth About Shrooms & Screening Panels

With rising interest in psychedelic therapy and the growing use of magic mushrooms (shrooms), questions around drug screening are becoming more common—especially for those in safety-sensitive jobs.

So, can psilocybin be detected in drug tests?
The short answer: yes—but not always. The truth depends on what kind of test is used, who’s ordering it, and how recently the substance was used.

Let’s dive into the science, policy, and employer realities behind shroom detection in 2025.

What Is Psilocybin and Why Is It Tested?

Psilocybin is a naturally occurring psychedelic compound found in several species of mushrooms. It’s being explored in clinical settings for treating depression, PTSD, and addiction.

However, as of 2025:

• Psilocybin remains a Schedule I controlled substance under federal law.

• Use is still illegal for DOT-regulated workers and in most corporate workplace settings.

Can Psilocybin Be Detected in Drug Tests?

Most standard drug tests (5-panel) used by employers and DOT do not test for psilocybin. But specialized labs can detect it—if they’re looking for it.

Here’s what matters:

• Detection is possible, but not automatic

• Employers must specifically request psilocybin in the drug panel

• Some extended drug tests include it

Detection Windows for Psilocybin

While DOT tests don’t include it by default, some scenarios where detection is likely include:

• Court-ordered drug screening (e.g., DUI or child custody cases)

• Return-to-Duty testing after a substance violation

• Private employer policies with zero-tolerance for psychedelics

• Rehab or clinical program evaluations

Will Employers Fire You If Psilocybin Is Detected?

If you’re working under DOT regulations, the answer is yes.

Even if not tested for initially, any:

• Admission of use

• Behavioral signs of impairment

• Positive screening from extended panels

…can result in:

• Immediate suspension

• Mandatory SAP Evaluation

• Return-to-Duty program before reinstatement

👉 Understand the SAP Process Here »

Is Psilocybin Legal in My State?

As of 2025:

• Oregon and Colorado have decriminalized or regulated psilocybin for therapeutic use

• Most states still treat it as illegal

• Federal law still applies, especially for CDL drivers and DOT-regulated workers

💡 Even in legal states, employers and the federal government do not have to tolerate its use.

What to Do If You’re Required to Take a Drug Test

If you’re worried about a possible detection:

1. Review the panel type (is it 5-panel or extended?)

2. Don’t assume “not tested” means “safe to use”

3. If flagged or referred, complete your SAP Evaluation and Return-to-Duty steps the right way

👉 Start a DOT-Compliant SAP Evaluation Here »

Final Thoughts: Can Psilocybin Be Detected in Drug Tests?

Yes—if the test is designed to detect it.
No—if you’re taking a basic employment screen.
But don’t let that mislead you. Even suspicion, disclosure, or self-reported use of psilocybin can have serious employment consequences, especially in DOT-regulated industries.